rabbit anti titin  (Proteintech)

Journal: bioRxiv

Article Title: Mechanobiological Specialization of Choroid Plexus Macrophages Defined by Titin Expression

doi: 10.64898/2026.01.20.700716

Figure Lengend Snippet: Titin-expressing macrophages are present in the choroid plexus. (A) Heatmap of the top eight differentially expressed genes in each of the three ChP macrophage sub-clusters. Gene expression levels are represented on a normalized gradient scale. (B) DAB immunohistochemical stain of a 5 µm FFPE section of control ChP tissue using a CD163 antibody. CD163-positive macrophages are marked by brown staining, while cell nuclei are counterstained blue with hematoxylin, indicating their distribution within the vascularized ChP tissue. Scale bar = 40 µm. (C) Violin plots illustrating TTN expression levels in ChP samples from Alzheimer’s disease and control conditions. Each plot shows the distribution, density, and variability of TTN transcript counts in ChP macrophages within each condition. (D) Schematic representation of the experimental workflow for evaluation of TTN RNA enrichment in macrophages, involving tissue dissociation, CD163 positive cell enrichment, RNA isolation, cDNA synthesis, PCR amplification using isoform-specific TTN primers, and ΔΔCq calculation with GAPDH used for normalization. (E) Fold changes, computed from ΔΔCq, in TTN isoform expression relative to GAPDH in CD163-bead enriched macrophages relative to whole tissue. The x-axis represents different primer sets (1, 3, 7, and 12), and the y-axis shows fold changes (log scale). Each point represents a single donor sample/primer set pair. (F) Schematic representation of the TTN gene with highlighted primer binding sites, with genomic coordinates on chromosome 2, indicating selected splicing sites and isoforms of TTN. (G) Immunofluorescence images of ChP with anti-CD68 (red) and anti-titin (green) primary antibodies, co-stained with DAPI (blue), showing cytoplasmic titin protein in ChP macrophage cytoplasm. Merged images are in right column. Scale bars = 20 µm.

Article Snippet: For titin immunofluorescence, primary antibody incubation with rabbit anti-titin (Proteintech Catalog #27867-1-AP) at 1:250 dilution and mouse anti-CD68 antibody (Millipore Cat.#168M-95) at 1:250 was conducted overnight at 4°C, followed by detection with secondary donkey anti-rabbit Alexa Fluor 488 (Thermo Fisher Scientific, Cat. #A21206) (1:500) and goat anti-mouse Alexa Fluor 647 (Thermo Fisher Scientific, Cat. #A32728), with nuclei counterstaining with DAPI.

Techniques: Expressing, Gene Expression, Immunohistochemical staining, Staining, Control, Isolation, cDNA Synthesis, Amplification, Binding Assay, Immunofluorescence

Journal: STAR Protocols

Article Title: Protocol for in vivo and ex vivo assessment of hyperglycemia and islet function in diabetic mice

doi: 10.1016/j.xpro.2023.102133

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-Ki67 (1:100) , Novus Biologicals , Cat# NB600-1209; RRID: AB_10001641.

Techniques: Plasmid Preparation, Recombinant, Blocking Assay, Saline, Fluorescence, SYBR Green Assay, Staining, Software

Journal: eLife

Article Title: Tension-driven multi-scale self-organisation in human iPSC-derived muscle fibers

doi: 10.7554/eLife.76649

Figure Lengend Snippet: ( A ) Rhodamine-phalloidin stainings of days 3 and 5 myofibers seeded at 2.5, 5, 10, and 20 k/cm 2 , pseudo-coloured according to local fiber orientation by OrientationJ (orientation colour-coding as in ). ( B ) Nematic correlation length η L of conditions shown in A , n=5 each. ( C ) Immunofluorescence against titin N-terminus for conditions shown in A . ( D ) Average autocorrelation function (ACF) for conditions shown in C , n=15 each. ( E ) Amplitude of secondary ACF peaks for conditions shown in D , n=15 each. Scale bars: 500 µm in A , 20 µm in C . Multiple pair-wise t-test. Source data available for B, E . Figure 5—source data 1. Table containing source data from .

Article Snippet: Antibody , Rabbit-anti-titin (N-term) , Sigma, Cat. HPA007042 , , 1 in 50.

Techniques: Immunofluorescence

Journal: eLife

Article Title: Tension-driven multi-scale self-organisation in human iPSC-derived muscle fibers

doi: 10.7554/eLife.76649

Figure Lengend Snippet:

Article Snippet: Antibody , Rabbit-anti-titin (N-term) , Sigma, Cat. HPA007042 , , 1 in 50.

Techniques: Derivative Assay, Sequencing, Knock-Out, Software